1994 Volume 38 Issue 1 Pages 7-12
Extra-band was occasionally detected in beta-fraction on cellulose acetate electrophoresis, especially when electrophoresis was done immediately after the replacement of bridging filter paper and the electrophoresis buffer. We have also noticed that the application of Toyo filter paper No. 2 as the bridging material, develops the extra-band in beta-fraction and increases the relative value of the beta-fraction, in the first run after the replacement of bridging filter paper. It has been reported that the presence of double-peak in beta-fraction on Tiselius' free electrophoresis, when electrophoresis buffer with high concentration of Ca ion was used. Therefore, we measured Ca, Mg and Fe concentrations of the eluate from two kinds of filter paper, and found relatively high concentration of Ca in the eluate from the Toyo filter paper No. 2. Immuno-fixation electrophoresis revealed that the C3 shifts from beta-fraction to slow beta-fraction and form extra-peak, when high concentration of Ca ion was exist in the electrophoresis buffer. In order to obtain more precise data on protein fractionation by cellulose acetate electrophoresis, it is essential to pay much attention on the selection of filter paper for bridging.
