Abstract
DNA binding proteins play important role in the DNA replication, recombination, transcription and the viral integration to genome. Several methods to analyse these DNA binding proteins such as methylation interference and DNase protection analysis were developed. Among them gel shift analysis has been widely used because of its simplicity, high sensitivity and rapidity. We review the detail of this method by refering to the example of the analysis of the redox regulation of the transcription factor NF-κ B binding to DNA. In this analysis the DNA binding activity of the NF-κB was abolished by the treatment with the oxidizing reagent diamide. Thioredoxin (ADF), but not glutathione efficiently activated NF-κB binding activity to its consensus DNA sequence.
