Abstract
Phospholipase D (PLD) was highly purified from cabbage leaves of three different cultivars. Each purified PLD showed a single protein band with molecular masses of 87 kDa on SDS-PAGE, respectively. Peptide maps of each purified PLD after digestion with Lysyl Endopeptidase or V8 Protease gave the same pattern of proteolytic peptide fragments. Previously, Pannenberg et al. suggested the existence of two isozymes designated as PLD1 and PLD2. The N-terminal amino acid sequences of whole PLD and of proteolytic peptide fragments were determined. As a result, the PLD isolated from cabbage leaves was identified as PLD2.
