Evidence by genotyping and identification of apo E5 and E7 by multiple banding patterns

Abstract

Atypical bands are frequently found at cathode side than the band of apolipoprotein (apo) E4 isoforms separated by Phenotyping Apo E IEF System (Jokoh Co. Ltd.). Then, we developed to resolve the atypical phenotype bands as apo E5 and apo E7 isoforms by PCR with restriction fragment length polymorphism and with amplification refractory mutation system. From the results, the genetic identification of atypical apo E5 and apo E7 phenotype bands was well accorded to the apo E phenotypes separated by the Phenotyping Apo E IEF system.