Abstract
We devised a simple and sensitive method for the determination of oligoclonal IgG bands (OCB) in the cerebrospinal fluid (CSF) by combining agarose gel electrophoresis with immunoblotting. In this method, it is not neccessary to concentrate the CSF. This method is effective for the separation of proteins present at low concentrations, such as is CSF samples. Its specificity is excellent. We were able to clearly detect OCBs in CSF samples, even when the IgG concentration was low, and also when there was strong masking by a polyclonal IgG smear. Our method is concluded to be useful for identification of OCBs in CSF samples.
