Abstract
Liposome immunoassays have been studied extensively, since the introduction by Kinsky et al. The mode of the assay, releasing entrapped signal-generating materials from liposomes, is based on destabilization of liposomal membrane. The quantity of released signal-generating materials is proportional to the concentration of analytes. Monitoring is generally by spectrophotometric and fluorimetric methods. However, as far as we know, few investigations have been made with regard to dyestuff-containing liposomes as a labeling reagent in immunoassay. In 2000 we proposed an immunoassay using chemiluminescence detection of dyestuff-containing liposomes as a labeling reagent. The immunoassay features high sensitivity, a small sample volume, and easy and rapid operation. In this report, the new immunoassay using capillary electrophoresis-chemiluminescence detection of dyestuff-containing liposomes as a labeling reagent is shortly introduced together with their background.
