Abstract
The stability of serum lipoproteins in vitro was described. Serial changes in the appearance of serum during 37°C heating were investigated. In agent-free serum samples, turbidity decreased 5 and 10 days after heating was started, but then serially increased. In V.E or EDTA added serum samples, there were no changes until 15 days after heating was started. Turbidity in creased 20 days after heating was started. Serial changes in lipoproteins on polyacrylamide gel disk electrophoresis (PAGE) were investigated. The appearance of LDL subfractions, appearance of IDL, LDL shift to positive electrodes, and decrease in HDL were observed. When serial changes in lipid parameters were investigated, stable values were obtained in V.E or EDTA added serum samples. EDTA effectively prevented lipoprotein degeneration, suggesting that serum metals are closely involved in oxidation degeneration. It was also confirmed that serum lipoproteins showed findings other than oxidation degeneration in vitro.
