Abstract
1) High voltage paper electrophoresis was used for the quantitative analysis of the free amino acids of human blood serum.
2) The serum (1.0ml) was deproteinized by adding methanol-acetone (3:1) mixture and the supernatant was concentrated to 0.1ml in water bath. Electrophoresis was performed at a potential gradient of 100 volt/cm and in acetic acid-pyridine buffer (pH 3.5). The time of electrophoresis was 12 and 60 minutes.
3) The amino acid patterns of 22 normal human sera hardly showed any individual difference.
4) The amino acid patterns of gastric ulcer, cholelithiasis and tuberculosis of the lung nearly agreed with that of normal human blood serum.
5) The blood serum of patient with carcinoma gave various different amino acid patterns, but these patterns were classified by five types on carcinoma of the stomach and three types on carcinoma of the breast. It was found that these types of amino acid patterns were related to the grade of developements of carcinoma.
6) Banti's syndrome showed a particular type of amino acid pattern which was found in other diseases.
