Paper Electrophoretic Studies on Proteins
1961 Volume 7 Issue 4 Pages 167-179
Details
1961 Volume 7 Issue 4 Pages 167-179
About the filterpaper electrophoresis of proteins, we come to a conclusion as the following, from the result of experiment, ussing serum (plasma) proteins as trial material after studing the provision of experimentation, material of experimentation according to the method of the experimentation.
1. We find inportant condition in the position on which we put the trial material, how much to have an overdose of it, and how fresh to have it.
2. The quality of the filterpaper has a close relation with the result of electrophoresis of proteins. We find the good result in the filterpaper of Whatman No. 1, Schleicher & Schüll No. 2043 a, b or Toyo filterpaper corp. No. 51, 51a.
3. About to the condition of the electrophoresis, the voltage, the electric current and the time of the phoresis are concerned each other. As to decide strictly on these point, we find some deficult problem, but we know that the condition of the phoresis had decided naturally, if we have the fixed filterpaper and buffer.
4. We find the best conditioh in about 7cm in phoresis distance and in 100-110°C, 20 minutes in the method of the desiccate as the same as we find from the result of experiment as to the phoretic distance and the desiccate method.
5. 20 minutes are the best in the time of dyeing so for as Amido black 10B is concermed.
We think as if We will find some deference between Amido black 10B and B. P. B. dyeingin in the result of the some amount, but it is not only clear above mentioned writting in this point of view but it is also necessary to study in future.
