Abstract
We reexamined safety of some cosmetic materials on a hereditary toxicity. The cosmetic materials tested were preservatives, Sunscreens, antioxidants, hormones and surfactants. Mutagenicity assay were carried out for Rec-assay using Wild and Recombination-deficient strains of B. Subutilis and Reversion assay using Salmonella typhimurium TA98 and TA100 with or without the microsomal activation (±S-9mix). In addition to the above-described procedures, Rec-assay with irradiating fluorescent lump or cold incubation were carried out to some materials, such as sunscreens and antioxidants. Irg-DP300, a preservative, was weak rec-positive at very high concentration such as 5 mg/ disk, but negative on revession assay. Both 8-MOP and 0-201 newly found out as positive control of photo-rec assay were clearly rec-positive by irradiation of fluorescent lump, but all of sunscreens and antioxidants were negative. Estradiol, a hormone, induced slightly more revertants than control with S-9mix and three anion surfactants of sulfate derivatives (Surf-1, 2, 4) and two nonion surfactants of cetyl ether and lanolin alcohol derivatives (Surf-13, 14) also exhibited the same effects with or without S-9mix, but we couldn't sufficiently recognize these mutagenicity. These phenomena are yet obscure whether due to any reasons.
