Abstract
It is reported that squalene hydroperoxide, which is the primary peroxide of squalene, is produced by exposing skin to sunlight. Using squalene hydroperoxide as a marker, we studied inhibitory effects of some antioxidants on lipid peroxide production of sunlight exposed skin. Each ethyl-acetate solution of purified squalene and an antioxidant mixture was applied on the forehead skin, and exposed to sunlight. By specific detection of squalene hydroperoxide using the chemiluminescence-high performance liquid chromatography system (CL-HPLC), the peroxide value of exposed samples could be determined. dl-α-Tocopherol was more effective than BHT.
