Application of MTT Test for the Screening of Cell Growth Activators Evaluation of α-Hydroxy Acids

Abstract

It has been reported by Von Scott that α-hydroxy acids have potent effects on wrinkle-repairing. MTT (3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium Bromide) method has been used for estimation of cytotoxicity, mitogenic activity and cell viability. We attempted the development of the cell activity estimating method by using MTT method and the investigation of α-hydroxy acids-wrinkle repairing mechanism on human dermis fibroblast culture system. It has been found that glycolic acid increased the activity which was estimated by the MTT method. Subsequently the proliferation effect of glycolic acid was examined. Glycolic acid showed high proliferating activity.
Furthermore, the effect on collagen synthesis was examined.
However, the enhancement on collagen synthesis by glycolic acid was not found.
From these results, it was speculated that the wrinkle-repairing of glycolic acid was realized by the enhancement of proliferation in dermal fibroblasts.