Scavengiug Activities of Polyhydroxybenzoic Acids on Hydroxyl Radicals

Abstract

The relationship between photoaging skin which is characterized by deep wrinkles and pig mentation, and active oxygen species has been studied by many researchers.
We had detected hydroxyl radicals in murine fibroblasts during UVB irradiation.
In this study the scavenging activities of polyhydroxybenzoic acid derivatives on such hydroxyl radicals were evaluated using ESR-spin trapping and human fibroblasts culture system.
Gallic acid showed the highest activity among chemicals tested in the both methods of ESR-spin trapping and the cell culture system.
Furthermore, alkyl esters of gallic acid were evaluated on the protective activity against hydroxyl radicals-induced cell damage. Stearyl gallate showed the potent protection against the celldamage in situ and in pretreating.