Studies for Analyzing the Prohibited Ingredients Such as Vitamin L1 and Pilocarpine Hydrochloride in Cosmetics

Abstract

We have already reported the analytical method of procaine hydrochloride and dibucaine hydrochloride (prohibited in cosmetics on the public comment of Ministry of Health and Welfare of Japanese Government in 1986). In order to find out another prohibited ingredients in cosmetics, we estimated the analytical method of vitamin L1 and pilocarpine hydrochloride by HPLC. Vitamin L1 and pilocarpine hydrochloride in the lotions or milky lotions were extracted with methanol. The extract was analyzed by HPLC using the column (Shiseido CAPCELL PAK C₁₈, 4.6×250mm) and a mixture of acetonitrile and 50mM phosphate buffer (pH 5) (37:3) or (19:1) for vitamin L1 and pilocarpine hydrochloride were used. The detection wavelength of vitamin L1 and pilocarpine hydrochloride were at 244 and 214nm, respectively. By the use of the above analytical method we made it sure that vitamin L1 and pilocarpine hydrochloride could be determined without the interference of the ingredients.