Abstract
Molecular cytogenetic characterization of the 5S ribosomal DNA (rDNA) was performed in a lynx spider Oxyopes sertatus. The 5S rDNA repeat sequence was determined by PCR-based cloning and subsequent sequence analysis: one unit was approximately 3670 bp in length, of which 3550 bp, a non-transcribed spacer (NTS), contained a full length of the histone H2B-like gene attached with the 5' flanking region essential for the transcription. This introduction of histone H2B-like gene into 5S rDNA cluster had never been reported in previous studies. Then, we mapped the chromosomal locations of the 5S rDNA by fluorescence in situ hybridization (FISH), and identified sites on three different chromosomes. We discuss on the linkages of 5S rDNA with multigene families including histone H2B-like gene.
