2013 Volume 16 Issue 3+4 Pages 47-50
Karyotype and distribution of C-band-positive constitutive heterochromatin and silver-positive nucleolus organizer regions (Ag-NORs) were studied in an anemonefish, Amphiprion perideraion. This species had the diploid chromosome number of 48 consisting of 6 pairs of metacentric, 13 pairs of submetacentric and 5 pairs of subtelo- or acrocentric chromosomes, and the fundamental number was calculated as 86. The karytotype of this species was very similar to those of A. clarkii and A. frenatus already reported, but C-bands and Ag-NORs showed different distribution among the three species. The present study demonstrated that C-bands and Ag-NORs were useful markers to distinguish very similar karyotypes of the three anemonefishes.
