SEISAN KENKYU
Online ISSN : 1881-2058
Print ISSN : 0037-105X
ISSN-L : 0037-105X
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A separation method with length-dependent mobility of long DNA molecules in nanofluidic device
Beomjoon KIMKyungduck PARK
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2012 Volume 64 Issue 3 Pages 307-311

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Abstract
We propose a new fabrication method for nanofluidic device with novel shapes using thermal shadow evaporation and wet anisotropic etching in aqueous KOH solution. We also demonstrate that a single DNA molecule could be driven in the nanofluidic device by electrophoresis, in which the linear stretching and electrophoretic migration of a DNA polymer molecule could be observed at a single molecule level. Finally, we investigate length-dependent mobility of chromosome-sized DNA in nanofluidic channel and successfully separate different lengths of DNA under simultaneous action of electric field as well as pressure gradient.[This abstract is not included in the PDF]
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© 2012 Institute of Industrial Science The University of Tokyo
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