Abstract
We propose a new fabrication method for nanofluidic device with novel shapes using thermal shadow evaporation and wet anisotropic etching in aqueous KOH solution. We also demonstrate that a single DNA molecule could be driven in the nanofluidic device by electrophoresis, in which the linear stretching and electrophoretic migration of a DNA polymer molecule could be observed at a single molecule level. Finally, we investigate length-dependent mobility of chromosome-sized DNA in nanofluidic channel and successfully separate different lengths of DNA under simultaneous action of electric field as well as pressure gradient.[This abstract is not included in the PDF]
