1988 Volume 29 Issue 4 Pages 155-161
Using the phosphate-free detergents formulated with protease and lipase, the effects of stability of protease in the washing solution on removal of protein soils from cotton fabric were investigated at pH9.5, 30°C.
1) Effect of PVA on stabilities of protease activity in the solutions containing anionic surfactant; DBS, was recognized markedly, but on the removal of gelatin from cotton fabric was not so effective compared with stabilized activity.
2) Addition of Ca2+ to protease was recognized more effectively than addition of PVA not only on activity but also on gelatin soil removal.
3) In the washing liquor containing various compounds, protein soil removal by hydrolysis was influenced by adsorption of protease-compound complex.
4) In the model formulations of detergent, protease/lipase/DBS systems, protein in mixed soil with oils and fats was removed effectively not only by the actions of hydrolysis by protease but also by lipase hydrolysis because of synergistic effects.
