Abstract
We report here the effect of lauryl alcohol (LA), an effective organic solvent for in situ extraction for production of paclitaxel, on the production of taxanes (10-deacetyl baccatin III, baccatin III and cephalomannine) in a suspension callus culture of Taxus cuspidata. The culture conditions for enhancing production of the taxanes by avoiding feed-back inhibition of paclitaxel were examined. Increased callus growth and production amounts of the taxanes except cephalomannine were obtained in the LA-medium culture systems compared to the control culture where LA was not added. This result suggested that LA influenced the cellular and/or enzymatic activities involved in the biosynthetic pathway of paclitaxel.
