Bonding Adhesion of Soft Tissue to Dentin Using TAD-Albumin glue

Abstract

Many adhesives with high bonding strength have been developed and applied in the biomedical field. These adhesives, however, have been used only for bonding between soft tissues. We developed a novel tissue adhesive (TAD-Albumin glue) consisting of tartaric acid derivative (TAD) and human serum albumin (HSA). The TAD-Albumin glue hardens by the amide bonding of HSA and TAD. It has been confirmed that the TAD-Albumin glue can combine with collagen fibers in the same way. Therefore, the TAD-Albumin glue, after being applied properly to exposed collagen fibers on the root surface, could mediate the binding between connective tissue and root surface. This study was designed to examine the bonding activity of the TAD-Albumin glue applied between dentin and soft tissue, and to evaluate its biocompatibility in vivo. To examine whether tooth surface treatment affects the bonding strength or not, human dentin chips were treated with saturated citric acid or 10% EDTA for 1, 3, and 5 min, and others were non-treated. Treated or non-treated dentin chips were bonded to columnar resin with stationery adhesive. Then the TAD-Albumin glue was applied to the dentin chip surface, and collagen-coated columnar resin was located on the chips. The bond strength was measured with a tensile machine. For histological examination, the TAD-Albumin glue was applied to the dentin chips treated with saturated citric acid for 3 min, then the chips were subcutaneously inserted into the back of forty 10 week-oldmale Wistar rats. To evaluate biocompatibility, the dentin chips were removed together with surrounding tissues on 0, 3, 7, 21 and 42 days after operation, and prepared for hematoxylin and eosin (H-E) sections for histological observation. The experimental group treated with saturated citric acid for 3 min had significantly higher bond strength than the non-treated group (p<0.0l). Histologically, the TAD-Albumin glue maintained bonding activity for 7 days after operation. No remarkable inflammatory cell infiltration was observed around the dentin chips. In conclusion, this study suggests that: 1. Citric acid treatment increased the bond strength of TAD-Albumin glue. 2. The TAD-Albumin glue maintained bonding activity for 7 days without tissue damage.