2013 Volume 56 Issue 4 Pages 291-297
Purpose: It is important to maintain gingival health, which is directly influenced by periodontal disease pathogenesis, in order to prevent the development and progression of periodontal disease. There have been several reports on the effect of gingival massage. Isotropic high-density carbon has high far-infrared radiation efficiency, and is expected to be useful for various medical devices. However, there has been no report on the influence of isotropic high-density carbon on the gums. In the present study, we investigated the effect of a carbon roller made from isotropic high-density carbon on a normal human gingival fibroblast cell line (Gin-1). Methods: A carbon roller was used as a heating (37 or 42℃) and vibrating massage roller (Gum-roller, Ohgi Technological Creation). Isotropic high-density carbon (carbon ball) was used at the tip of the roller. Gin-1 was cultured in 10% FBS in DMEM. A carbon ball group, carbon ball+heating (37 or 42℃)+vibrating group, and control group (no stimulation by carbon roller) were fabricated to evaluate the effect of the carbon roller. Consequently, FGF-2, NGF, VEGF, and EGF were produced using real-time PCR and ELISA, and cell activity was evaluated by a WST assay and morphological changes were examined by microscope using Giemsa staining. Results: FGF-2 mRNA expression of Gin-1 significantly increased in the carbon roller+heating (37℃)+vibrating group compared with the control group. VEGF and EGFmRNA were not detected. FGF-2 protein production by Gin-1 also increased in the carbon roller+heating+vibrating group. Conclusion: The results suggest that the carbon roller used in the present study increased FGF-2 production by gingival fibroblasts, and contributed to the improvement of gingival wound healing.
