2013 Volume 56 Issue 4 Pages 325-334
Purpose: The purpose of this study was to monitor the main periodontopathic bacteria during the treatment of periodontal diseases and to re-evaluate the effectiveness of monitoring of bacteria. Methods: Sixteen patients (6 males and 10 females, mean age 40.6) with chronic periodontitis (CP) or aggressive periodontitis (AP) participated in this study. Probing pocket depth, clinical attachment level, bleeding on probing, Plaque Index, and suppuration of all existing teeth were undertaken for each patient. One deep pocket site (DS, PD ≥4 mm) and one shallow pocket site (CS as control, PD<3 mm) were chosen in each patient. Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Treponema denticola (Td) and Aggregatibacter actinomycetemcomitans (Aa) were detected in each subgingival pocket by means of the real-time PCR method. Clinical measurements and the bacterial examination were done in the baseline, before scaling and root planning (SRP), and one month and two months after SRP. Results: Seven CP and nine AP patients were examined. Of these, 7 patients completed all the examinations. At the baseline, the distribution of bacteria, except Aa, tended to be higher in the deep pocket sites of CP than in AP In CS, bacteria tended to be high when Plaque Index ≥1. Aa was only found in 1 AP patient. Pg and Tf tended to be detected even in shallow pockets and persistently existed after SRP, particularly when Pl I was higher. However, Td was detected only in deep pockets and rapidly disappeared after SRP. In addition, Pg and Tf tended to recover faster in the patients with poor oral hygiene. In the patients treated by full mouth-SRP in conjunction with systemic antimicrobial therapy, all examined bacteria became negative 2 months after SRP. Conclusion: The examination and monitoring of periodontopathic bacteria seemed to be effective to diagnose periodontal diseases and to make and re-evaluate treatment plans.
