The Biological Effects of Bonding Systems on Lymphocytes Using an in vitro Model

Abstract

Purpose: Although high success rates of direct pulp capping using resin bonding systems have been reported, damage to subjacent pulp lymphocytes has never been discussed. The purpose of this study was to evaluate the effects of bonding systems on mouse spleen lymphocytes (MSL). Methods: The bonding systems used included a self-etching primer and a bonding monomer (Clearfil SE Bond, Kuraray Medical). MSL were adjusted to a 5×10⁶ (cells/ml) concentration and placed into a modified micro-centrifuge tube. Simulated pulp exposure was created on the tube lid with a 16 G needle. A drop of primer and adhesive was poured into the exposure and light-cured. A drop of saline was used as a control specimen. Reacted MSL were collected from the top, middle, and bottom (corresponding to the root apex) of each tube. The survival rates of MSL were determined by a hemacytometer. Results: The results indicated that the cell survival rate of the primer plus bonding specimen was significantly lower than that of other specimens at both the top and bottom areas (n=5, p<0.01, ANOVA). Conclusion: It is suggested that the primer used in this study may have adverse effects on lymphocytes.