2015 Volume 58 Issue 5 Pages 391-397
Purpose: The periodontal ligament is present between hard tissue; the ligament is constantly under mechanical stress and is not calcified to maintain homeostasis despite having a hard-tissue-forming ability. The homeostasis of the periodontal ligament is said to exist and the ratio of TGF-β1 and BMP-2 is involved. PLAP-1 is known as a protein which controls the BMP-2, but the protein which controls the TGF-β1 is not clear. Therefore, we calculated the ratio of the amount of gene expression using microarray for cultured normal human periodontal ligament fibroblasts (hPdLF) and cultured normal human gingival fibroblasts (Gin-1), the ratio of the expression level, especially in hPdLF for high leucine significance in the periodontal ligament was not a clear leucine rich repeat containing 32 (LRRC32), tissue-derived culture cells adjacent to the periodontal ligament in order to clarify the specificity in the periodontal tissue (Gin-1, normal human dermal fibroblasts (NHDF), normal human osteoblasts (NHOst), and cultured human epithelial cells (HeLa) ) were compared and examined using RT-PCR.
Materials and methods: Cultured hPdLF and Gin-1 from total RNA were extracted and analyzed by microarray. RT-PCR is hPdLF, Gin-1, NHDF, NHOst and HeLa from extracted and purified total RNA, and cDNA obtained by reverse transcription, GAPDH as an internal standard LRRC32, and is an indicator of calcification, Periostin, osteocalcin (OC), osteopontin (OPN), RUNX2, bone sialoprotein (BSP) and TGF-β1 by PCR.
Results: In analysis by microarray, LRRC32 was found that is strongly expressed in hPdLF compared to Gin-1. The gene expression of LRRC32 in hPdLF of three different lots by RT-PCR was observed and was derived from soft tissue Gin-1, NHDF, NHOst, and HeLa gene expression in LRRC32 was not recognized. On the other hand, gene expression of LRRC32 was observed in NHOst from hard tissue. Expression of the gene of a marker of calcification OPN, OC, RUNX2, Osterix gene expression was observed in hPdLF, OC, RUNX2, Osterix gene expression was observed in NHOst.
Conclusion: LRRC32 is constitutively expressed in the periodontal ligament, suggesting that it is expressed in tissues involved in hard tissue formation.
