Effects of Bioactive Glass Based Sealer on Cell Migration Ability and Viability of Periodontal Ligament Cells and Osteoblast-like Cells

Abstract

 Purpose: For endodontic treatment, biocompatibility of root canal filling sealer is essential. In the present study, we examined the effects of a newly developed bioactive glass based sealer (BG based sealer) on cell migration ability and viability to clarify the biocompatibility of this sealer.

 Methods: Human periodontal ligament cells (HPDLC) and osteoblast-like cells (MC3T3-E1) were respectively cultured in 24-well Transwell plates. Transwell culture inserts including sealers (eugenol based, non-eugenol based, or BG based sealers) were inserted into the wells, then cell migration ability and viability were analyzed by wound healing assay and trypan blue staining.

 Results: In the wound healing assay, migration ability of HPDLC under eugenol based sealer was not analyzed because of cell death. Under eugenol based sealer (quick), non-eugenol based sealer, or BG based sealer, HPDLC showed migration ability, but the ability was suppressed compared with the control. MC3T3-E1 under eugenol based sealer showed cell survival but not migration. Under eugenol based sealer (quick), non-eugenol based sealer, or BG based sealer, MC3T3-E1 showed no significant difference of cell migration in comparison with the control. In trypan blue staining, eugenol based sealers significantly suppressed the viability of both cells, while non-eugenol based and BG based sealers showed no effects on the viability of both cells, and they increased time-dependently.

 Conclusion: BG based sealer showed suppressive effects but did not inhibit the migration ability of HPDLC, and showed no significant inhibitory effects on the viabilities of both cells, suggesting that BG based sealer has high biocompatibility.