2018 Volume 61 Issue 6 Pages 354-360
Purpose: To investigate the antimicrobial effects of surface pre-reacted glass ionomer (S-PRG) filler eluate containing several ions including fluoride, strontium and boron on polymicrobial biofilm grown from stimulated saliva.
Methods: Stimulated saliva was obtained from a healthy donor. S-PRG eluate was obtained by suspending S-PRG filler in distilled water. Polymicrobial biofilm was grown under anaerobic conditions at 37℃ on glass coverslips suspended for 10 h in buffered McBain 2005 medium containing the saliva, then in fresh medium without saliva for a further 14 h. They were then suspended under anaerobic conditions for a further 24 h in buffered McBain 2005 medium with either S-PRG eluate containing 110.5 ppm fluoride (the PRG group), NaF solution at 110.5 ppm F (the NaF group) or nothing (the Cont group). Colony-forming units (CFU) were counted at 48 h, 0 h being the start of cultivation, and statistically analyzed (one-way ANOVA, Games-Howell, p<0.05). Also at 48 h, the total amounts of bacteria in the biofilms and amounts of Streptococcus, Actinomyces and Veillonella identified by their 16S rRNA were analyzed.
Results: CFU was lower in the PRG group than in the Cont and NaF groups. Total 16S rRNA level was lower in the PRG group than in the Cont and NaF groups (Cont: 8.24×109, NaF: 4.68×109, PRG: 8.30×108). There was no difference in total 16S rRNA levels between the Cont and NaF groups. The 16S rRNA levels of Streptococcus, Actinomyces and Veillonella were lower in the PRG group than in the other two groups (Streptococcus: Cont: 2.10×109, NaF: 1.19×109, PRG: 3.83×108, Actinomyces: Cont: 8.69×106, NaF: 3.27×106, PRG: 4.23×105, Veillonella: Cont: 2.06×109, NaF: 1.90×109, PRG: 7.58×107).
Conclusion: Ions in S-PRG eluate not only suppressed total viable bacteria in the biofilms but also the initial colonizers of the biofilms.
