2020 Volume 63 Issue 2 Pages 156-164
Purpose: We evaluated the enamel remineralizing potential of a calcium-fluoroaluminosilicate glass-based desensitizer (Nanoseal) on enamel subsurface lesions in vitro by using micro-focus X-ray computed tomography (μCT).
Methods: Extracted human molars were sectioned mesiodistally and buccolingually into four specimens per molar. Specimens were immersed in a demineralizing solution (pH 4.3) for 10 days to create enamel subsurface lesions. The specimens were then randomly divided into the following four groups: DW (Milli-Q water) as a negative control, and NS (Nanoseal), NaF9000 (sodium fluoride [NaF] 9,000 ppm) and NaF900 (NaF 900 ppm) as positive controls. In the NS group, the two solutions that made up the NS material were mixed, and the coating was applied to the test surface for 20 s. In the other groups, the solutions were applied to the test surface for 20 s. After each treatment, the specimens were rinsed off with a mild air-water spray for 15 s and then immersed in artificial saliva (pH 7.0) for 10 days. μCT scans of all specimens were obtained before demineralization, after demineralization, and after remineralization. Mineral density (MD) and mineral loss (ΔZ) of enamel subsurface lesions were measured by μCT. The percentage of remineralization (%R) was calculated from ΔZ.
Results: The specimens in all the test groups showed mineral recovery in the subsurface lesions after remineralization. However, in the DW and NaF900 groups, parts beneath the enamel surface were clearly still demineralized, in comparison with the NS and NaF9000 groups. From the mean MD profiles, the NS group showed the greatest remineralization within all parts of lesions. %R was highest in the NS group (p<0.05). The remineralization of the DW as a negative control, and NaF900 and NaF9000 groups as positive controls did not differ significantly (p>0.05).
Conclusion: According to the results of this study, a calcium-fluoroaluminosilicate glass-based desensitizer increases the remineralization of artificial human enamel subsurface lesions.
