Endogeneous modulation for mineralization of pulp tissue

Abstract

Human pulp tibroblasts (HPF-C) were isolated from deciduous teeth and cultured in vitro. With continued culture in normal tissue culture medium, six pulp fibroblast strains formed mineralized nodules. HPF-C showed high levels of alkaline phosphatase activity that could be increased more than seven fold by the addition of 1,25 (OH)_2D_3 at 5-50×10^<-9>. In addition to the production of type I collagen, these cells also synthesized fibronectin and osteonectin, and expressed mRNA of transforming growth factortype B and liver / bone / kidney alkaline phosphatase. The responses of HPF-C to parathyroid hormone, cAMP and calcitonin were similar to that of odontoblasts. Also, HPF-C in contact with hydroxyapatite served as a mediator of cell-to-cell interaction and was related to the functional differentiation of cells for tissue repair, including pulpal calcification.