Affinity of artificial bone implant material in human osteoblast-like cells

Abstract

I cultured the cell line MG-63, which is derived from human osteosarcoma, and retains osteoblast characteristics, with and without hydroxyapatite (HAP). I examined the histocytochemistry, biosynthesis of the extracellular matrix of fibronectin, type I and type III collagen, and osteocalcin synthesis as a function of mineralization. An ACAS570, laser cytometer revealed that after one week of culture fibronectin caused the osteoblasts to adhere securely to the HAP surface. In addition, type I and type III collagen were synthesized after about two to three weeks. Synthesis of the extracellular matrix and subsequent mineralization were both substantially enhanced in the culture cells around of the HAP. These results suggest that the HAP implant surface may act as a substrate to permit cell growth that improves bone formation at bone defects.