Effect of Fluorosurfactant on the Adsorption of Protein to Synthetic Hydroxyapatite

Abstract

     I determined physico-chemically and electro-chemically the surface characterization of synthetic hydroxyapatite (HAp) and dental enamel treated with fluorosurfactant (FSA), and the resulting adsorption of protein. The HAp was treated with the FSA that had four different types of polar hydrophilic radicals (anionic, nonionic, amphoteric and cationic).
     The surface characterizations of the FSA-treated HAp were analyzed for several parameters: surface tension, zeta potential, surface chemical composition and contact angle. The results indicated that treatment of FSA resulted in surface alterations of HAp and dental enamel. In particular, the contact angles of dental enamel treated with anionic or nonionic FSA indicated a slight increase in hydrophobicity compared with untreated dental enamel, while treatments with amphoteric or cationic FSA significantly increased hydrophobicity of the dental enamel surface.
     The adsorption of protein (HSA, CA and PR) on the surface of HAp treated with FSA was analyzed quantitatively by zeta potential. The adsorption decreased with treatment of anionic FSA, nonionic FSA and cationic FSA (DS-202), and was not influenced by treatment with amphoteric FSA and cationic FSA (Lodyne and Zonyl).
     These results indicate that the adsorption of protein to the sorbent is greatly influenced by the hydrophobicity of the sorbent surface.