Physicochemical changes in glycoproteins caused by phospholipids and hydrogen ion diffusion

Abstract

We studied the changes caused by phospholipids (phosphatidylethanolamine (PE) and phosphatidyl-serine (PS)) in the physicochemical properties and hydrogen ion diffusion of glycoprotein. Six types of glycoprotein were injected into the sample port and hydrogen ion diffusion was measured. A fluorescent probe with 8-anilino-1-naphthalene sulfonate was used to determine changes in the hydrophobicity and surface potential of the above glycoprotein. The permeability of the glycoprotein membrane was inversely related to the concentration of phospholipids in the glycoprotein layer. It was found that permeability of glycoprotein associated with PS is lower than that of glycoprotein associated with PE. PS and PE had different hydrophobicity and surface potential. These findings suggest that phospholipids in the glycoprotein layers play an important role in retarding hydrogen ion diffusion, creating increased hydrophobicity and maintaining surface potential in the glycoprotein layer.