Growth characteristics of cells cultured from different areas of the human periodontal ligament

Abstract

We evaluated the growth characteristics of cells derived from the bone side (BP cells), middle (MP cells) and cementum side (CP cells) of the human periodontal ligament (PL). The PL cells were cultured from the mandibular right third molar and first premolar. Cultured cells were grown in Dulbecco's modified Eagle medium containing various concentrations (5, 10 and 20%) of fetal bovine serum (FBS), and the number of cells was counted at 5 and 10 days. The growth of the CP cells was the lowest of all examined. The BP, MP and CP cells showed different growth patterns for each of the three FBS concentrations. These results suggested that different areas of the PL have different proliferative responses.