Effect of combined regional hyperthermochemotherapy with CDDP on human oral carcinoma transplanted in nude mice

Abstract

     We investigated the effect of combined regional hyperthermochemotherapy with cis-diamminedichloroplatinum (CDDP) on human oral carcinoma (KB cells) transplanted subcutaneously in the leg of BALB/cA nude mice. Upon obtaining tumors of about 8 mm diameter, the following treatments were performed. Hyperthermia was carried out in a water bath at 39, 41, 43 and 45°C for 30 minutes. CDDP was given intraperitoneally at 1, 3, 5 and 7 mg/kg of body weight. Single or combined treatments were performed on days 0, 3 and 6, and tumor size and animal weight were measured. The relative mean tumor weight (RW) compared with day 0 and the relative mean body weight (RBW) compared with day 0 were calculated. The relationship of hyperthermochemotherapy and individual anti-tumor effects were compared by the ratio of the relative mean tumor weights for the treatment and control groups. The potentiation ratio to the effect of CDDP with hyperthermia was calculated for each temperature.
     The results were as follows:
1. Regional hyperthermochemotherapy seemed to have a potential inhibitory effect on the growth of the KB cells.
2. The anti-tumor effect of CDDP with hyperthermia at 43°C was nearly six fold that at 39°C.
3. There were no marked side effects in the regional hyperthermochemotherapy group.
     We concluded that combined regional hyperthermochemotherapy with CDDP is an effective treatment.