Adsorption of human salivary proteins to synthetic hydroxyapatite

Abstract

I carried out biochemical and physicochemical investigations on the adsorption of salivary proteins to synthetic hydroxyapatite (HAp) using salivary proteins fractionated from whole human saliva by gel filtration. Correlation between the adsorption volume of protein and the zeta potential of HAp indicated that at low concentrations the salivary protein adsorbed on the HAp surface with a monolayer of the Langmuir type, and with a multi-layer at high concentrations. The residues of basic and hydrophobic amino acids decreased in the shlivary protein solution after addorption. These results indicate that the adsorption of salivary proteins to HAp is dependent on electrostatic and hydrophobic interaction.