Effect on New Attachment Formation when Tecth Are Reimplanted after Exposure to a Spinner Culture of Periodontal Ligament Cells

Abstract

We examined whether application of a long spinner culture of periodontal ligament cells to the root surface before reimplantation is capable of creating new attachment. Premolars were extracted from a monkey and cells from the periodontal ligaments were cultured. The maxillary central incisors were then extracted, the cementum and pulps removed, and the root canals filled. After culturing the periodontal ligament cells on the planed root surface of the right central incisor, the tooth was maintained in a spinner culture for 4 weeks. The left central incisor, which was preserved without exposure to the cells, served as a control. The teeth were then reimplanted to their original sockets. After 6 weeks the seeded root surface was covered with fibrillar tissues. However, epithelial downgrowth, root resorption and ankylosis were observed in the control. These results indicate that this new technique may promote the formation of new connective tissue attachment.