Effect of the storage media used during tooth replantation on human periodontal ligament cells in vitro

Abstract

I evaluated the effect of ViaSpan, sterile saline, human resting saliva, and pasteurized milk as storage media for tooth replantation on intra-cellular LDH activity, growth activity, and morphological changes in human periodontal ligament cells. ViaSpan and pasteurized milk were superior to sterile saline and human resting saliva in their effect on intra-cellular LDH activity and cell growth activity. Human resting saliva had the greatest influence on morphological changes among the four media. There was correlation between the osmolality o f the storage media, intra-cellular LDH activity, and growth activity o f the periodontal ligament cells.