1996 Volume 59 Issue 4 Pages 301-312
Phagocytic cells play an important role in host defense mechanisms against bacterial infection. Gram negative bacteria are often isolated from periodontal pockets of patients with periodontal disease. Changes in the function of polymorphonuclear leukocytes (PMN) and macrophages (Mφ), which may be induced by lipopolysaccharide (LPS) and bacterial components, contribute to the pathogenesis of periodontal disease. There are also phagocytic cells in the gingival pockets of patients with periodontal disease. The hypoxic condition of these pockets promotes increases in the number of gram negative anaerobes.
I investigated the effect of LPS and dissolved oxygen concentration (DO) on the chemotaxis and phagocytosis of mouse PMN and MΦ. Using the hot-phenol water method, I extracted LPS derived from Porphyromanas gingivalis, Capnocytophaga ochrachea, Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans. DO was adjusted to 0.87, 1.35, 2.28 and 3.78 ppm in Dulbecco's modified eagle medium. LPS and decreases in DO significantly inhibited the function of PMN and Mφ. These results suggest that LPS and decreases in gingival pocket DO affect the functions of PMN and Mφ, and promote the pathogenesis of periodontal disease. Shika lgaku (J Osaka Odontol Soc)1996 Dec; 59(4): 301-312.
