In vitro induction of stress protein by chemicals in HeLa cells
1997 Volume 60 Issue 4 Pages 273-287
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1997 Volume 60 Issue 4 Pages 273-287
We evaluated the cellular reactions to ten chemicals including components of dental cements by examining stress protein synthesis and cell viability with neutral red(NR) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT). Induction of 70, 000-Da stress protein (HSP 70) synthesis in HeLa cells by chemicals was evaluated by an enhanced chemiluminescence system.
HSP 70 synthesis was induced during the early stage between three and six hours of exposure to phosphoric acid, methyl methacrylate monomer(MMA), formalin and hydrochloric acid at concentrations of 50 to 500μM. Acrylamide induced HSP 70 to a small extent, while the other agents examined, i.e. eugenol, itaconic acid, chloroform, acetone and xylene, had no effect. The results of NR and MTT assays revealed that phosphoric acid, eugenol, itaconic acid, formalin, hydrochloric acid and acrylamide at concentrations of 500μM and over, inhibited the cells, while MMA, chloroform, acetone and xylene did not.
It was concluded that the synthesis of HSP 70 might represent a cellular change caused by acidic chemicals, and that the concentration that induced HSP 70 synthesis was lower than that inhibiting cell viability. Shika Igaku (J Osaka Odontol Soc) 1997 Dec； 60（4）：273-287．
