Molecular biological study of cytokeratin CK 19 gene in oral squamous cell carcinoma

Abstract

In order to reserch the role of cytokeratin (CK) 19 gene expression in carcinogenesis, I investigated the effect of the expression of CK 19 gene in oral squamous cell carcinoma, epithelial dysplasia and normal gingiva. Total-RNA was extracted from biopsy materials and examined by revers transcription polymerase chain reaction (RT-PCR) and northern blot. The results showed that CK 19 mRNA expressed in every sample, and it was strongly positive in squamous cell carcinoma, moderately positive in epithelial dysplasia and slightly positive in normal gjngiva. These results jndicafes that CK 19 gene is an important factor of carcinogenesis in oral squamous cell epithelia.