1998 Volume 61 Issue 3_4 Pages 196-197
Although it is reasonable to believe that alteration of occlusion may cause or exacerbate temporomandibular disorders, the exact role of occlusion is still unknown. I immunohistochemically examined the changes that occurred after tooth extraction in rat mandibular condylar cartilage. Unilateral maxillary molars were extracted from rats, and the temporomandibular joints were resected after 1, 2, 3 or 6 months. The tissues were fixed with paraformaldehyde, embedded in paraffin after decalcification with EDTA, and then prepared in serial sections for immunohistochemjcal examination for types I, II and X collagen, chondroitin 4-sulfate, chondroitin 6-sulfate, keratan sulfate and dermatan sulfate proteoglycan. In the experimental rats, staining for types I and X collagen, C4S, KS and DSPG was stronger, whereas that for C6S was weaker, than in the controls. These findings seem to indicate a loss of elasticity in the mandibular condylar cartilage.