Estrogenic activity of dental materials and bisphenol-A related chemicals in vitro

Abstract

We tested 28 materials for estrogenicity using three in vitro bioassays. Several of these were dental materials and the others were bisphenol-A-related chemicals. Bisphenol-A(BPA), bisphenol-F(BPF) and bisphenol-A-bischloroformate(BPACF) showed estrogenic activity by the yeast two-hybrid system, and BPA, BPF, BPACF and bisphenol-S(BPS) showed estrogenic activity by the fluorescence polarization system. However, none of the remaining chemicals and none of the dental materials showed any activity at concentrations between 10^<-3>M and 10^<-7>M. Although BPA, BPF, BPACF, bisphenol-A-dimethacrylate and BPS showed estrogenic activity by MCF-7 cells in the E-screen test, the remaining chemicals did not. Further studies should be done on the relationship of structure and activity, and on the in vivo activity of the materials.