Influence of periodontal dressing materials on tubule-like structure formation by umbilical vein endothelial cells

Abstract

We did an in vitro study using the co-culture technique on the influence of periodontal dressing materials on the formation of tubule-like structures by human umbilical vein endothelial cells and human diploid fibroblasts of dermal origin. The rate of growth in length and in area were measured for the tubule-like structures using an Angiogenesis Image Analyzer (Kurabo Industries Ltd., Osaka, Japan). Cell viability was examined by 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide (MTT). Of the three materials tested, Coe-Pak, which is eugenol-free, had the largest values for both tests. Formation of the tubule-like structures occurred in the range of 0.078-5.000 mg/mL. In contrast, Surgicalpack N, which was in the eugenol group, had the lowest, and Peripac was in between the other two. Results for the cell viability test were similar to those for the formation of tubule-like structures. We found that eugenol-type periodontal dressing materials had a greater inhibitory effect than the eugenol-free type. This co-culture technique has not previously been used for this type of study. Further studies of this type should be productive. Shika Igaku (J Osaka Odontol Soc) 2003 Jun; 66(2): 167-175.