Tissue compatibility of a newly-developed 4-META/MMA-TBB resin-based reverse filling material

Abstract

We examined the tissue compatibility, sealing ability and interface between a newly-developed resin-based reverse filling material and the root canal wall using scanning electron microscopy (SEM). The filling material was composed of 4-META/MMA-TBB and zirconium oxide (RO). The sealing ability of RO was better than that of the control material (amalgam) when evaluated by apical dye leakage. SEM observation of the interface between RO and the canal wall showed resin tags and hybridized dentin. Histopathological examination (H-E stain) of rat subcutaneous tissues revealed that inflammatory responses were less than with the control material. Likewise, infiltration of macrophages seen by immunohistochemical staining was also less than in the control. We found that RO was less irritating to the periapical tissue than was the control material. These results indicate that the newly developed reverse filling material may be useful for endodontic treatment.