2 Three-dimensional structure and bone regeneration process of bovine periosteal cells

Abstract

I investigated the bone regeneration process using bovine periosteal cells by examining the effect of ascorbic acid, mRNA expression of specific markers and the ultrastructure of osteoblasts. Cells cultured in ascorbic acid, had a three-dimensional structure at a concentration of 50μg/mL ascorbic acid, but not at concentrations of 10μg/mL or 0μg/mL. mRNA expression of type 1 collagen, osteocalcin and alkaline phosphatase were observed after three weeks. These results seem to indicate that the three-dimensional structure of cells and differentiation into osteoblast-like cells are important in the bone regeneration process.