6 Growth of human gingival fibroblasts and Ca 9-22 human gingival epithelial cells on the surface of composite resin (in vitro)

Abstract

Human gingival fibroblasts and epithelial cells were cultured on the surface of composite resin to investigate the influence of restorative materials on gingival tissue. Composite resins were either immersed in PBS for three days or not subjected to immersion. The specimens were then either polished with #600 or #10000 SiC abrasive paper, and some of them were not polished. Cell growth was determined using the MTS assay. ELISA assay was used to determine fibronectin and vitronectin on the resin surface, and the expression of integrin β_1. We concluded that cell growth on the surface of immersed or polished composite resin was greater than non-immersed or non-polished specimens, respectively.