2007 Volume 70 Issue 1 Pages 65-71
We investigated primary cultured cells derived from three cases of follicular type ameloblastoma, in order to search for a new therapy for this disease. We used to immunohistochemical methods and reverse transcriptase-polymerase chain reaction (RT-PCR) to examine whether ameloblastoma-like cells treated with or without ratinoic acid and vitamin D3 would differentiate.
The primary cultured cells derived from ameloblastoma, spread in monolayers or double layers of ameloblastoma-like cells having nuclei that stained with hematoxillin. Proliferating cell nuclear antigen (PCNA) was observed in the perphery of the ameloblastoma colony. Ratinoic acid (RA) induced upregulation of the transcription level of amelogenin andenamelin mRNA. Vitamin D3 (VD) induced upregulation of the translation of cytokeratin.
These results suggest that the cells derived from ameloblastoma differentiate to more mature cells when induced by ratinoic acid and vitamin D3.
