Effect of retinoic acid on differentiation of follicular ameloblastoma-derived cells

Abstract

We used Western blotting and protein antibody array (AB array) to examine protein changes induced by vitamin A at the time of increased amelogenin and enamelin mRNA in ameloblastoma-like cells. Western blotting showed that vitamin A induced upregulation of Notch and Sonic hedgehog (SHH) proteins. AB array showed that the upregulation induced by vitamin A decreased Ras-GRF2 and Rho-GDI, while it increased TNFα and TRADD compared with the controls. These results indicated that the vitamin A acted to control ameloblastoma-like cell redifferentiation and inhibited growth.