1 Cell survival and gene expression under compressive stress in a three-dimensional in vitro human periodontal ligament-like tissue model

Abstract

We studied the cell survival and gene expression of three-dimensional in vitro human periodontal ligament-like tissue (HPdLLT) under various compressive stress conditions mimicking orthodontic force. HPdLLTs were subjected to 5, 15, 25, and 35 g/cm^2 compressive stress for 1, 3, 7 or 14 days. Similar extracellular matrix, spindle-shaped cells and its survival inside or on the surface of each in vitro HPdLLT with no relation to compressive stress duration or intensity. Genes showed their distinctive expressions according to compressive stress duration or intensity. This study indicated that in vitro HPdLLT effectively mimicked the reaction and gene expression of human PDL in response to orthodontic force.