2015 Volume 78 Issue 1 Pages 17-18
The purpose of this study was to evaluate the Chondrocyte differentiation potential of dedifferentiated fat cells (DFATs) and adipose stem cells (ASCs) from the buccal fat pad (BFP) in an atelocollagen sponge. The gene expression levels of Chondrocyte differentiation markers were higher in DFATs than in ASCs cultured for 14 and 21 days, whereas protein expression levels were higher in DFATs at all time points tested for aggrecan. Additionally, the levels of the embryonic stem cell markers were higher in DFATs than in ASCs at 72 h. The extracellular matrix of both the cultured ASCs and DFATs was Alcianblue-positive and was also aggrecan-positive. Therefore, we think that BFP-derived DFATs are an ideal cell source for cartilage tissue engineering.
