Effects of synthetic oligopeptide derived from enamel matrix derivative on cell proliferation, cell attachment, and activation of the signal of extracellular signal­regulated kinase(ERK)1/2 in human gingival fibroblasts

Abstract

Enamel matrix derivative (EMD) is used in periodontal tissue regeneration therapy. However, patients may avoid EMD because it is animal­derived and may include pathogenic or­ ganisms. We evaluated a synthetic oligopeptide (SP) derived from EMD that we produced in previous basic research of EMD to determine whether it contributes to wound healing in peri­ odontal tissue. We also investigated the efficacy of SP derived from EMD on human gingival fi­ broblasts (HGF). The HGF were treated with SP at 0, 1, 10, 100 and 1000 ng/mL. We examined the effects of SP on cell proliferation and the capacity for cell attachment in HGF. We also in­ vestigated the role of extracellular signal­related kinase (ERK) 1/2 induced by SP. Our results suggested that SP significantly promotes cell proliferation and cell attachment in HGFs. More­ over, SP also induced ERK 1/2 activation in HGF. The results also suggested that SP promotes cell proliferation and the activity of cell attachment in HGF. Therefore, SP might contribute to the promotion of wound healing in periodontal tissue. Shika Igaku (J Osaka Odontol Soc) 2017 ;Mar ;80(1) : 1­7.